Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System
We are trying to elucidate the role of ubiquitination in neuronal development. Using a biochemical approach, we have developed a novel proteomic strategy that allows isolation of ubiquitinated material from the brain of a living organism. So far, we are using Drosophila for this purpose. We have also applied the same approach to isolate SUMO conjugates in a tissue specific manner.
M. Franco, N. T. Seyfried, A. H. Brand, J. Peng and Ugo Mayor. A novel strategy to isolate ubiquitin conjugates reveals wide role of ubiquitination during neural development. Molecular & Cellular Proteomics (2011), 10(5):M110.002188 (Epub on 22nd September 2010) PMID: 20861518
U. Mayor and J. Peng. Deciphering Tissue-specific Ubiquitylation by Mass Spectrometry. Methods in Molecular Biology (2012), 832: 65-80
In vivo requirements of the UBL modifier SUMO on SALL proteins function during steroidogenesis: D. melanogaster as a model system.