Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System

Severine Boulon

Education

2005 : PhD (University of Montpellier, France)

1998 : BSc in Biochemistry, Paris 7 University, Paris, France

Research Appointments

Since 2010 : Maître de Conférences (Assistant Professor) at the University of Montpellier, France

2005 – 2010 : Post-Doctoral Fellow at the Centre for Gene Regulation and Expression (Prof . A.I. Lamond), Dundee, UK

Severine Boulon

Research Interests

 

-       Establishing the role of a nuclear regulator of the 20S core proteasome, PA28g, in the response of cells to DNA damage, in the dynamics of nuclear organization and in the resistance of leukemia cells to chemotherapy

 

-       Identifying new targets of PA28g by SILAC quantitative proteomics

 

-       Investigating the role of a newly identified protein, PIP30, in the regulation of PA28g activity

 

 

Selected Publications

 

¨ Cioce M., Boulon S., Matera A.G., Lamond A.I. (2006). J Cell Biol. 175(3):401-13. UV-induced fragmentation of Cajal bodies.

¨ Boulon S, Marmier-Gourrier N, Pradet-Balade B, Wurth L, Verheggen C, Jády BE, Rothé B,   Pescia C, Robert MC, Kiss T, Bardoni B, Krol A, Branlant C, Allmang C, Bertrand E, Charpentier  B. (2008). J Cell Biol. 11;180(3):579-95. The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.

¨ Trinkle-Mulcahy L, Boulon S, Lam YW, Urcia R, Boisvert FM, Vandermoere F, Morrice NA, Swift S, Rothbauer U, Leonhardt H, Lamond A. (2008). J Cell Biol 20;183(2):223-39. Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes.

¨ Boulon S, Ahmad Y, Trinkle-Mulcahy L, Verheggen C, Cobley A, Gregor P, Bertrand E, Whitehorn M, Lamond AI. (2010). Mol Cell Proteomics. May;9(5):861-79. Establishment of a protein frequency library and its application in the reliable identification of specific protein interaction partners.

¨ Boulon S, Pradet-Balade B, Verheggen C, Molle D, Boireau S, Georgieva M, Azzag K, Robert MC, Ahmad Y, Neel H, Lamond AI, Bertrand E. (2010) Mol Cell. Sep 24;39(6):912-24. HSP90 and its R2TP/Prefoldin-like cochaperone are involved in the cytoplasmic assembly of RNA polymerase II.

¨ Boulon S, Westman BJ, Hutten S, Boisvert FM, Lamond AI. (2010) Mol Cell. Oct 22;40(2):216-27. The nucleolus under stress.

¨ ten Have S, Boulon S, Ahmad Y, Lamond AI. (2011) Proteomics. Mar;11(6):1153-9. Mass spectrometry-based immuno-precipitation proteomics - the user's guide.

¨ Boulon S, Bertrand E, Pradet-Balade B. (2012) RNA Biol. Feb 1;9(2):148-54. HSP90 and the R2TP co-chaperone complex: building multi-protein machineries essential for cell growth and gene expression.

 

BOOK CHAPTER:

¨ Boulon S.  (February 2, 2012). Book "Integrative Proteomics" edited by Hon-Chiu Eastwood Leung, Subject editors: Tsz-Kwong Man and Ricardo J. Flores , ISBN 978-953-51-0070-6, InTech. Dynamics of Protein Complexes Tracked by Quantitative Proteomics.

Partner

CRBM-CNRS