Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System

Koraljka Husnjak

Education

  • 1991 - 1997: BSc in molecular biology, Faculty of Science and Mathematics, University of Zagreb, Croatia.
  • 1997 - 2001: MSc in molecular biology, Faculty of Science and Mathematics, University of Zagreb, Croatia.
  • 2001 - 2005: PhD in molecular biology, Faculty of Science and Mathematics, University of Zagreb, Croatia.
  • 2005 - 2010: Postdoctoral Fellow with Prof. Ivan Dikic, Institute of Biochemistry II, Goethe University Medical School, Frankfurt am Main, Germany.

Research Appointments

  • 1997 - 2001: MSc student, Rudjer Boskovic Institute, Zagreb, Croatia.
  • 2001 - 2005: PhD student, Rudjer Boskovic Institute, Zagreb, Croatia.
  • 2005 - 2009: Postdoctoral Fellow, Institute of Biochemistry II, Goethe Medical School, Frankfurt am Main, Germany.
  • 2010 - present: junior group leader, Institute of Biochemistry II, Goethe Medical School, Frankfurt am Main, Germany.
Koraljka Husnjak

Research Interests

We are interested in the role of ubiquitin and various ubiquitin-binding domains (UBDs) during growth and development.

We are currently focusing on the role of intrinsic proteasomal ubiquitin receptor Rpn13, which we are studying in a mouse model, as well as by using various molecular biology tools.

Figure 1. Pru domain of ubiquitin receptor Rpn13 binds ubiquitin by “loops”that capture ubiquitin.

Figure 2. Confocal immunofluorescent analysis of HeLa cells using ubiquitin antibody. The cells were treated with puromycin for 2 hours before fixation, leading to the accumulation of ubiquitin-positive aggregates.

Selected Publications

Grabbe C, Husnjak K, Dikic I (2011) The spatial and temporal organization of ubiquitin networks. Nat Rev Mol Cell Biol 12(5), 295-307

Husnjak K*, Elsasser S*, Zhang N*, Chen X, Randles L, Shi Y, Hofmann K, Walters K, Finley D, Dikic I (2008) Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 22, 453 (7194), 481-8

Schreiner P*, Chen X*, Husnjak K*, Randles L, Zhang N, Elsasser S, Finley D, Dikic I, Walters K, Groll M (2008) Ubiquitin docking at the proteasome via a novel PH domain interaction. Nature 22, 453 (7194), 548-52

Schmidt MH*, Husnjak K*, Szymkiewicz I, Haglund K, Dikic I (2006) Cbl escapes Cdc42-mediated inhibition by downregulation of the adaptor molecule betaPix. Oncogene 25(21), 3071-8

Kowanetz K*, Husnjak K*, Höller D, Kowanetz M, Soubeyran P, Hirsch D, Schmidt MHH, Pavelic K, De Camilli P, Randazzo PA, Dikic I (2004) CIN85 associates with multiple effectors controlling intracellular trafficking of EGF receptors. Mol Biol Cell 15, 3155-66

Project

The role of proteasomal Ub receptor Rpn13 in mouse development.