Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System
We are interested in the role of ubiquitin and various ubiquitin-binding domains (UBDs) during growth and development.
We are currently focusing on the role of intrinsic proteasomal ubiquitin receptor Rpn13, which we are studying in a mouse model, as well as by using various molecular biology tools.
Figure 1. Pru domain of ubiquitin receptor Rpn13 binds ubiquitin by “loops”that capture ubiquitin.
Figure 2. Confocal immunofluorescent analysis of HeLa cells using ubiquitin antibody. The cells were treated with puromycin for 2 hours before fixation, leading to the accumulation of ubiquitin-positive aggregates.
Grabbe C, Husnjak K, Dikic I (2011) The spatial and temporal organization of ubiquitin networks. Nat Rev Mol Cell Biol 12(5), 295-307
Husnjak K*, Elsasser S*, Zhang N*, Chen X, Randles L, Shi Y, Hofmann K, Walters K, Finley D, Dikic I (2008) Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 22, 453 (7194), 481-8
Schreiner P*, Chen X*, Husnjak K*, Randles L, Zhang N, Elsasser S, Finley D, Dikic I, Walters K, Groll M (2008) Ubiquitin docking at the proteasome via a novel PH domain interaction. Nature 22, 453 (7194), 548-52
Schmidt MH*, Husnjak K*, Szymkiewicz I, Haglund K, Dikic I (2006) Cbl escapes Cdc42-mediated inhibition by downregulation of the adaptor molecule betaPix. Oncogene 25(21), 3071-8
Kowanetz K*, Husnjak K*, Höller D, Kowanetz M, Soubeyran P, Hirsch D, Schmidt MHH, Pavelic K, De Camilli P, Randazzo PA, Dikic I (2004) CIN85 associates with multiple effectors controlling intracellular trafficking of EGF receptors. Mol Biol Cell 15, 3155-66
The role of proteasomal Ub receptor Rpn13 in mouse development.