Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System

Ivan Dikic

Education

  • 1986 – 1991: Medical Doctor, University of Zagreb Medical School, Summa cum laude.
  • 1991 - 1995: Ph.D. - Molecular Biology University of Zagreb and New York University under supervision of Prof. J. Schlessinger.
  • 1995 - 1997: Postdoctoral Fellow with Prof. Joseph Schlessinger, New York University Medical Center, New York, USA

Research Appointments

  • 1991 - 1992: International Institute for Mother and Child Health Care, Zagreb, Croatia.
  • 1992 - 1997: Research Associate at the New York University, NY, USA
  • 1997 - 2002: Group Leader, Ludwig Institute for Cancer Research, Uppsala, Sweden.
  • 2002 - 2008: C3 Professor, Institute of Biochemistry II, Goethe University, Medical School, Frankfurt, Germany.
  • 2002 - 2008: C3 Professor, Institute of Biochemistry II, Goethe University, Medical School, Frankfurt, Germany.
  • 2002 - present: Professor, School of Medicine University of Split, Croatia
  • 2009 - present: Director, Institute of Biochemistry II, Goethe University Medical School, Frankfurt am Main, Germany.
  • 2009 - present: Scientific Director of Frankfurt Institute for Molecular Life Sciences.
Ivan Dikic

Research Interests

We are interested in the role of ubiquitin and various ubiquitin-binding domains (UBDs) during growth and development.

We are currently focusing on the role of intrinsic proteasomal ubiquitin receptor Rpn13, which we are studying in a mouse model, as well as by using various molecular biology tools.

Figure 1. Pru domain of ubiquitin receptor Rpn13 binds ubiquitin by “loops”that capture ubiquitin.

Figure 2. Confocal immunofluorescent analysis of HeLa cells using ubiquitin antibody. The cells were treated with puromycin for 2 hours before fixation, leading to the accumulation of ubiquitin-positive aggregates.

Selected Publications

Grabbe C, Husnjak K, Dikic I (2011) The spatial and temporal organization of ubiquitin networks. Nat Rev Mol Cell Biol 12(5), 295-307

Husnjak K*, Elsasser S*, Zhang N*, Chen X, Randles L, Shi Y, Hofmann K, Walters K, Finley D, Dikic I (2008) Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 22, 453 (7194), 481-8

Schreiner P*, Chen X*, Husnjak K*, Randles L, Zhang N, Elsasser S, Finley D, Dikic I, Walters K, Groll M (2008) Ubiquitin docking at the proteasome via a novel PH domain interaction. Nature 22, 453 (7194), 548-52

Schmidt MH*, Husnjak K*, Szymkiewicz I, Haglund K, Dikic I (2006) Cbl escapes Cdc42-mediated inhibition by downregulation of the adaptor molecule betaPix. Oncogene 25(21), 3071-8

Kowanetz K*, Husnjak K*, Höller D, Kowanetz M, Soubeyran P, Hirsch D, Schmidt MHH, Pavelic K, De Camilli P, Randazzo PA, Dikic I (2004) CIN85 associates with multiple effectors controlling intracellular trafficking of EGF receptors. Mol Biol Cell 15, 3155-66

Fellow

Project

The role of proteasomal Ub receptor Rpn13 in mouse development.