Investigating the essential roles of Ubiquitin-like molecules and the Ubiquitin Proteasome System

Aude Echalier

Education

  • 2013: Habilitation à diriger des recherches. Université Montpellier I.
     
  • 2004: PhD in Biological Sciences at the University of Warwick, Coventry, UK.
  • 2001: MSc in Crystallography and NMR, Strasbourg and Nancy, France.

Research Appointments

  • 2014: Lecturer at the University of Leicester. Departments of Biochemistry and of Cancer Studies and Molecular Medicine.
  • 2009-2014: Lecturer at the Université Montpellier I and Group Leader at the Centre de Biochimie Structurale.
  • 2004-2009: Postdoctoral Fellow at the University of Oxford, Department of Biochemistry, UK.
Aude Echalier

 

Research Interests

We are interested in the role and regulation of CSN5 within and outside of the Cop9 signalosome. In particular, we are probing the interactions between CSN5 and cell cycle regulators.

We are using an integrated structural biology and bio-informatics approach to address these aspects.

Selected Publications

Birol, M., Enchev, R.I., Padilla, A., Stengel, F., Aebersold, R., Betzi, S., Yang, Y., Hoh, F., Peter, M., Dumas, C. and Echalier A. (2014). Structural and biochemical characterization of the Cop9 signalosome CSN5/CSN6 heterodimer. PLoS One. 2014 Aug 21;9(8):e105688.

Echalier, A., Hole, A.J., Lolli, G., Endicott, J.A. & Noble, M.E. (2014). An inhibitor's-eye view of the ATP-binding site of CDKs in different regulatory states. ACS Chem Biol. 9(6):1251-1256.

Birol, M. & Echalier, A. Structure and Function of MPN (Mpr1/Pad1 N-terminal) Domain-Containing Proteins. (2014). Curr Protein Pept Sci. 15(5):504-17

Carbain, B., Paterson, D.J., Anscombe, E., Campbell-Dexter, A., Cano, C., Echalier, A., Endicott, J., Golding, B.T., Haggerty, K., Hardcastle, I.R., Jewsbury, P.J., Newell, D.R., Noble, M., Roche, C., Wang, L.Z. & Griffin, R.J. 8-Substituted O6-Cyclohexylmethylguanine CDK2 Inhibitors; Using Structure-Based Inhibitor Design to Optimise an Alternative Binding Mode. (2014). J Med Chem. 57(1). 56-70

Echalier, A.), Pan, Y.*, Birol, M.*, Tavernier, N., Pintard, L., Hoh, F., Ebel, C., Galophe, N., Claret, F.-X., Dumas, C. Insights into the regulation of the human Cop9 signalosome catalytic subunit, CSN5/Jab1. (2013). PNAS. 110(4), 1273-1278. ) corresponding author; * equal contribution

Echalier, A.*, Cot, E.*, Camasses, A.*, Hodimont, E., Hoh, F., Jay, P., Sheinerman, F., Krasinska, L. and Fisher D. (2012). An integrated chemical biology approach provides insight into Cdk2 functional redundancy and inhibitor sensitivity. Chem Biol. 19(8):1028-1040.* equal contribution

Echalier, A., Endicott, J.A. and Noble, M.E. (2010). Recent developments in cyclin-dependent kinase biochemical and structural studies. Biochim Biophys Acta. 1804(3):511-519.

Takaki, T.*, Echalier, A.*, Brown, N.R.*, Hunt, T., Endicott, J.A. and Noble, M.E. (2009). The structure of CDK4/cyclin D3 has implications for models of CDK activation. Proc Natl Acad Sci U S A. 106(11):4171-4176.* equal contribution

Bettayeb, K., Sallam, H., Ferandin, Y., Popowycz, F., Fournet, G., Hassan, M., Echalier, A., Bernard, P., Endicott, J., Joseph, B. and Meijer, L. (2008). N-&-N, a new class of cell death-inducing kinase inhibitors derived from the purine roscovitine. Mol Cancer Ther. 7(9):2713-24

Bettayeb, K., Oumata, N., Echalier, A., Ferandin, Y., Endicott, J.A., Galons, H. and Meijer, L. (2008). CR8, a potent and selective, roscovitine-derived inhibitor of cyclin-dependent kinases. Oncogene. 27(44):5797-807.

Merckx, A.*, Echalier, A.*, Langford, K., Sicard, A., Langsley, G., Joore, J., Doerig, C., Noble, M. and Endicott, J. (2008). Structures of P. falciparum protein kinase 7 identify an activation motif and leads for inhibitor design. Structure. 16(2):228-38. * equal contribution

Echalier, A.*, Bettayeb, K.*, Ferandin, Y., Lozach, O., Clément, M., Valette, A., Liger, F., Marquet, B., Morris, J.C., Endicott, J.A., Joseph, B. and Meijer, L. (2008). Meriolins (3-(pyrimidin-4-yl)-7-azaindoles): synthesis, kinase inhibitory activity, cellular effects, and structure of a CDK2/cyclin A/meriolin complex. J Med Chem. 51(4):737-51. *Equal contribution

Bettayeb, K., Tirado, O.M., Marionneau-Lambot, S., Ferandin, Y., Lozach, O., Morris, J.C., Mateo-Lozano, S., Drueckes, P., Schächtele, C., Kubbutat, M.H., Liger, F., Marquet, B., Joseph, B., Echalier, A., Endicott, J.A., Notario, V. and Meijer, L. (2007). Meriolins, a new class of cell death inducing kinase inhibitors with enhanced selectivity for cyclin-dependent kinases. Cancer Res. 67(17):8325-34.

Griffin, R.J., Henderson, A., Curtin, N.J., Echalier, A., Endicott, J.A., Hardcastle, I.R., Newell, D.R., Noble, M.E., Wang, L.Z. and Golding, B.T. (2006). Searching for cyclin-dependent kinase inhibitors using a new variant of the cope elimination. J Am Chem Soc. 128(18):6012-3.

Echalier, A., Brittain, T., Wright, J., Boycheva, S., Mortuza, G.B., Fülöp, V. and Watmough, N.J. (2008). Redox-linked structural changes associated with the formation of a catalytically competent form of the diheme cytochrome c peroxidase from Pseudomonas aeruginosa. Biochemistry. 47(7):1947-56.

Pettigrew, G.W., Echalier, A. and Pauleta, S.R. (2006). Structure and mechanism in the bacterial dihaem cytochrome c peroxidases. J Inorg Biochem. 100(4):551-67.

Echalier, A., Goodhew, C.F., Pettigrew, G.W. and Fülöp, V. (2006). Activation and catalysis of the di-heme cytochrome c peroxidase from Paracoccus pantotrophus. Structure. 14(1):107-17.

Echalier, A., Glazer, R. L., Fülöp, V. and Geday, M. A. (2004). Assessing crystallization droplets contents using birefringence. Acta Cryst. D60, 696-702.

Echalier, A.*, Trivelli, X.*, Corbier, C., Rouhier , N., Walker, O., Tsan, P., Jacquot, J.P., Aubry, A., Krimm, I., Lancelin, J.M. (2005). Crystal structure and solution NMR dynamics of a D (type II) peroxiredoxin glutaredoxin and thioredoxin dependent: a new insight into the peroxiredoxin oligomerism. Biochemistry. 44(6):1755-67. * equal contribution

Josse D., Ebel C., Stroebel D., Fontaine A., Borges F., Echalier A., Baud D., Renault F., Le Maire M., Chabrieres E. and Masson P. (2002). Oligomeric states of the detergent-solubilized human serum paraoxonase (PON1). J. Biol. Chem. 277(36), 33386-97. Article publié à l’issue d’un stage estivale à la fin de ma maîtrise de biochimie

Brittain, T., Boyd, P.D.W., Fülöp, V. and Echalier, A. (2008). The mechanism of action of di-heme peroxidases: a theoretical study. Inorg. Biochem: Res. Prog. Chapter 8, 225-242. (Hughes. J.G. and Robinson A.J. Eds.), Nova Science, N.Y., U.S.A.